anti goat cd13  (R&D Systems)

Journal: bioRxiv

Article Title: Predicting recovery trajectories and injury severity following partial crush spinal cord injury in mice

doi: 10.64898/2026.02.28.708735

Figure Lengend Snippet: a. Representative survey IHC images of SCI lesions from the three AFS defined subgroups at 14 days after partial crush injury showing Gfap-positive astrocytes, Cd13-positive immune and stromal cells within the lesion core, and 5HT-positive descending serotonergic tract fibers. b. Gfap intensity plots for partial SCI mice stratified by subgroup. c. Quantification of total Gfap via an area under the curve (AUC) calculation of Gfap intensity from 500µm rostral to 500µm caudal of the lesion epicenter showing a subgroup dependent increase in total Gfap. ***p value < 0.0001, **p value < 0.002, One-way ANOVA with Tukey multiple comparison test. d. Quantification of extent of astrocyte bridging at SCI lesions stratified by subgroup, *p value < 0.02, One-way ANOVA with Tukey multiple comparison test. e. Cd13 intensity plots for partial SCI mice stratified by subgroup. f. Quantification of total Cd13 at SCI lesions at 3 and 14 days after SCI stratified by subgroup. *p value < 0.05, One-way ANOVA with Tukey multiple comparison test on 14d data g. Quantification of SCI lesion size at 3 and 14 days after SCI stratified by subgroup. **p value < 0.002, *p value < 0.05, One-way ANOVA with Tukey multiple comparison test on 14d data. Gfap and Cd13 intensity plots show mean as darken lines and s.e.m as shaded area. All graphs are mean ± s.e.m.

Article Snippet: The following primary antibodies were used in this study: anti-Rat Gfap (Invitrogen, 13–0300, 1:1000); anti-Guinea Pig Gfap (Synaptic Systems, 173 308, 1:500); anti-Goat Cd13 (R&D systems, AF2335, 1:500), anti-Rabbit 5-HT (Serotonin) (Immunostar, #20080, 1:1000); anti- Rat MBP (Sigma, MAB386, 1:500).

Techniques: Comparison

Journal: bioRxiv

Article Title: Predicting recovery trajectories and injury severity following partial crush spinal cord injury in mice

doi: 10.64898/2026.02.28.708735

Figure Lengend Snippet: a. Mean Open field (OF) locomotion scores for the saline and coacervate treated SCI mice over two weeks. **p value < 0.005, *p value < 0.05, One-way ANOVA with Tukey multiple comparison test. b. Grid walk (GW) test performance for the saline and coacervate treated SCI mice over two weeks, **p value < 0.0001, *p value < 0.02, One-way ANOVA with Tukey multiple comparison test. c. Correlation analysis of OF and GW scores for carrier injected SCI mice, with the behavioral tests showing strong positive correlation (r=0.86), p-value <0.0001, t-test for Pearson’s linear correlation. d. Pie charts indicating the AFS subgroup distribution across the saline and coacervate injected SCI mice. e. Mean OF, GW and combined assessment PMP values for the AFS designation separated by carrier injection type. f. Confusion matrix showing the percentage of mice displaying Class 1, 2 and 3 type recovery from the three AFS subgroups based on the combined evaluation of OF and GW testing results. g. Representative survey IHC images of carrier treated SCI lesions from the three AFS defined subgroups at 14 days after partial crush injury showing Gfap-positive astrocytes and Cd13-positive immune and stromal cells. h. Quantification of SCI lesion size at 14 days after SCI stratified by subgroup and carrier injection. *p value < 0.05, Two-way ANOVA with Tukey multiple comparison test. i. Quantification of extent of astrocyte bridging at SCI lesions stratified by subgroup and carrier injection., *p value < 0.02, Two-way ANOVA with Tukey multiple comparison test.

Article Snippet: The following primary antibodies were used in this study: anti-Rat Gfap (Invitrogen, 13–0300, 1:1000); anti-Guinea Pig Gfap (Synaptic Systems, 173 308, 1:500); anti-Goat Cd13 (R&D systems, AF2335, 1:500), anti-Rabbit 5-HT (Serotonin) (Immunostar, #20080, 1:1000); anti- Rat MBP (Sigma, MAB386, 1:500).

Techniques: Saline, Comparison, Injection

Journal: The European journal of neuroscience

Article Title: VEGF-E Attenuates Injury After Ischemic Stroke by Promoting Reparative Revascularization.

doi: 10.1111/ejn.70114

Figure Lengend Snippet: FIGURE 3 | Revascularization is stabilized by VEGF-E associated with attenuation of neuronal degeneration. (a) Representative fluorescence images of CD31 and PDGFRβ immunolabeling at the injury site in vehicle (VEH)- and VEGF-E-treated mice 4 days after stroke. (b) Analysis of the density of perivascular PDGFRβ+ cells at the injury site 4 days after stroke. (c) Analysis of CD31 and PDGFRβ colocalization at the injury site. (d) Analysis of the number of CD31+ microvascular stalls at the injury site. (e) Representative fluorescence images of CD13 and CD105 immunolabeling at the injury site in VEH- and VEGF-E-treated mice. (f) Analysis of the density of CD105+ microvessels at the injury site. (g) Analysis of CD105 and CD13 colocalization at the injury site. (h) Representative fluorescence images of FJB+ degenerating neurons in the ipsilateral cortex and ipsilateral striatum. Stereological analysis of the density of FJB+ degenerating neurons in the (i) ipsilateral cortex and (j) ipsilateral striatum. Data are boxplot with min/max or stacked histogram (n = 6–7 animals/group). *p < 0.05, **p < 0.01, ***p < 0.001 compared to control (b, d, f, i, unpaired two-tailed t- test). Abbreviations: IF, immunofluorescence; VEGF, vascular endothelial growth factor; VEH, vehicle.

Article Snippet: The following primary antibodies were used: rat anti- mouse cluster of differentiation (CD31) (1/500; BD Biosciences, ON, Canada; 550274), goat anti- mouse aminopeptidase N (CD13) (1/250; R&D systems), rat anti- mouse glial fibrillary acidic protein (GFAP) (1/500; Invitrogen, MA, USA; 13- 0300), rabbit anti- mouse ionized calcium binding adaptor molecule (IBA1) (1/500; WAKO, ON, Canada; 019- 19741), goat anti- rat CD45 (1/500; BD Biosciences; 553076), rabbit anti- mouse PDGFRβ (1/250; Abcam, ON, Canada; ab32570), rat anti- mouse endoglin (CD105) (1/250; Novus Biologicals, ON, Canada; NB100- 77666), and rabbit anti- mouse aquaporine- 4 (AQP4) (1/500; Cell Signaling Technology, MA, USA; 59678S).

Techniques: Fluorescence, Immunolabeling, Control, Two Tailed Test, Immunofluorescence